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| Phylogenetic Analysis of the Potential Microorganism for Remediation of Heavy Metals from the Contaminated Environment
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Source |
International Journal of Biometrics and Bioinformatics (IJBB) |
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Table of Contents |
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Volume: 3 Issue: 2 |
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Pages: 19-30 |
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Publication
Date: April 2009 |
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ISSN
(Online): 1985-2347 |
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Pages |
19 - 30 |
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Author(s) |
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Published
Date |
18-05-2009 |
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Publisher |
CSC
Journals, Kuala Lumpur,
Malaysia |
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ADDITIONAL
INFORMATION |
| Keywords Abstract References Cited by Related Articles Collaborative
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KEYWORDS: Bioremediation, Heavy metals, Bioinformatics |
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| The present research work has been carried out to study the waste disposal contaminated site for its physico chemical and microbial characterization and identification of potential microorganism capable of bioaccumulation and biodegradation of heavy metals. The ambient conditions present in the metal contaminated environment shows the values: pH(5.4),temperature(30°C), moisture(11.71%), nutrients; Nitrogen(0.2mg/l), phosphorus(22.65mg/l) and sulphur(559.3mg/l) respectively. The biological parameters studied indicate Dissolved oxygen (7.4mg/l), Biological oxygen demand (3.8 mg/l), Chemical oxygen demand (64.6 mg/l). The microbial consortium identified was found to survive and multiply in the present environmental conditions. Microbial consortium was sequenced and compared using Bioinformatics tools like BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentrations of heavy metals viz 5mg/l, 25mg/l, 50mg/l, 100mg/l up to 800mg/l with special reference to Iron. At a concentration of 500mg/l, only one microorganism was found survived and multiplied. This shows that potential microorganism was only survived at higher concentration of iron. The 16SrRNA sequence and phylogenetic tree characterized the organism as Klebsiella pneumoniae, which was also confirmed by biochemical tests. The potential microorganism identified by BLAST technique can be used for remediation of the heavy metal from contaminated environment. |
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| 1 |
APHA, Standard methods for examination of water and waste water. American Public Health Association, New York (1979).
|
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|
| 2 |
APHA, Standard methods for examination of water and waste water. American Public Health Association, New York (1989).
|
|
|
| 3 |
Bosshard PP, S Abels, R Zbinden, EC, Bottger, and M. Altwegg. 2003. “Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation)”. J. Clin. Microbiol, 41:4134–4140.
|
|
|
| 4 |
Chao Kuo-kuang, Chao chen-ching and Chao wei-liang.2003. “Suitability of the traditional microbial indicators and their enumerating methods in the assessment of fecal pollution of subtropical fresh water environmental”. Journal of microbiological and immunological infections, Vol. 36. pp. 288-293.
|
|
|
| 5 |
Bosshard PP, S Abels, R Zbinden, EC, Bottger, and M. Altwegg. 2003. “Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation)”. J. Clin. Microbiol, 41:4134–4140.
|
|
|
| 6 |
Chao Kuo-kuang, Chao chen-ching and Chao wei-liang.2003. “Suitability of the traditional microbial indicators and their enumerating methods in the assessment of fecal pollution of subtropical fresh water environmental”. Journal of microbiological and immunological infections, Vol. 36. pp. 288-293.
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| 7 |
Chaudhari, T., Fulekar, M.H., Eapen, S.2009.“Characterization of Industrial Waste and Identification of Potential Microorganism –Degrading Tributyl Phosphate” , Journal of Toxicology and environmental Health Sciences (In press).
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| 8 |
Choudhury, P., Kumar, R. “Multidrug- and metal-resistant strains of Klebsiella pneumoniae isolated from Penaeus monodon of the coastal waters of deltaic Sundarban”. Can J Microbiol, 44:186–189. 1998.
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| 9 |
Collins, C.H. and P.M. Lyne. “Microbiological Methods”. 5th Edition. Butterworth and Co (Publishers) Ltd (1985).
|
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|
| 10 |
EPA.1997. Electrokinetic laboratory and field processes to radioactive and hazardous mixed waste in soil and ground water. Washington, D.C. EPA 402/R-97/006.
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| 11 |
Fulekar, M.H. and J. Sharma. 2008. “Bioinformatics Applied in Bioremediation”. Innovative Romanian Food Biotechnology. Vol. 2 No. 2. pp 28-36.
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| 12 |
Fulekar, M.H. “Bioinformatics – Application in Life & Environment Sciences”, Springer publication. (2008).
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| 13 |
Fulekar, M.H. (a). “Bioremediation technologies for environment”. Indian Journal for Environmental Protection. 25(4): 358-364. (2005)
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| 14 |
Fulekar, MH. (b). “Environmental Biotechnology”. Oxford and IBH publishing house, New Delhi, India. (2005)
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| 15 |
Klinge, K. 1960. “Differential techniques and methods of isolation of pseudomonas by k. Klinge”. J. appl. Buct. 23 (3), 442-462.
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| 16 |
Kumar, H. 2004. “Recent trends in biotechnology”. Agrobios, India. pp. 55-58.
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| 17 |
Lone, M.I., Zhen-Li, H.E., Stofella, P.J. and Xiao, E.Y. 2008. “Phytoremediation of heavy metals polluted soils and water: progress and perspectives”. Journal of Zhejiang University Sci.B.9 (3): 210-220.
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| 18 |
Sharma, P.K., Balkwill, D.L., Frenkel A, Vairavamurthy, M.A. 2000. “A new Klebsiella planticola starin (Cd-1) grows anaerobically at high cadmium concentrations and precipitates cadmium sulfide”. Appl Environ Microbiol. 66(7): 3083–3087.
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| 19 |
Westhead, D.R., Parish, R.M., Twyman. Bioinformatics-Instant Notes. Bios Scientific Publisher.UK. (2003)
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www.YellowDocuments.com
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| Jaya Sharma : Colleagues
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| Madhusudan Fulekar : Colleagues
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