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Phylogenetic Analysis of the Potential Microorganism for Remediation of Heavy Metals from the Contaminated Environment
Jaya Sharma, Madhusudan Fulekar
Pages - 19 - 30     |    Revised - 05-05-2009     |    Published - 18-05-2009
Volume - 3   Issue - 2    |    Publication Date - April 2009  Table of Contents
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KEYWORDS
Bioremediation, Heavy metals, Bioinformatics
ABSTRACT
The present research work has been carried out to study the waste disposal contaminated site for its physico chemical and microbial characterization and identification of potential microorganism capable of bioaccumulation and biodegradation of heavy metals. The ambient conditions present in the metal contaminated environment shows the values: pH(5.4),temperature(30°C), moisture(11.71%), nutrients; Nitrogen(0.2mg/l), phosphorus(22.65mg/l) and sulphur(559.3mg/l) respectively. The biological parameters studied indicate Dissolved oxygen (7.4mg/l), Biological oxygen demand (3.8 mg/l), Chemical oxygen demand (64.6 mg/l). The microbial consortium identified was found to survive and multiply in the present environmental conditions. Microbial consortium was sequenced and compared using Bioinformatics tools like BLAST, ClustalW and PHYLIP. In order to identify potential microorganism, microbial consortium was exposed to increasing concentrations of heavy metals viz 5mg/l, 25mg/l, 50mg/l, 100mg/l up to 800mg/l with special reference to Iron. At a concentration of 500mg/l, only one microorganism was found survived and multiplied. This shows that potential microorganism was only survived at higher concentration of iron. The 16SrRNA sequence and phylogenetic tree characterized the organism as Klebsiella pneumoniae, which was also confirmed by biochemical tests. The potential microorganism identified by BLAST technique can be used for remediation of the heavy metal from contaminated environment.
CITED BY (5)  
1 Singh, R., Pathak, B., & Fulekar, M. H. (2015). Characterization of PGP Traits by Heavy Metals Tolerant Pseudomonas putida and Bacillus safensis Strain Isolated from Rhizospheric Zone of Weed (Phyllanthus urinaria) and its efficiency in Cd and Pb Removal. Int. J. Curr. Microbiol. App. Sci, 4(7), 954-975.
2 Colin, V. L., Villegas, L. B., & Abate, C. M. (2012). Indigenous microorganisms as potential bioremediators for environments contaminated with heavy metals. International Biodeterioration & Biodegradation, 69, 28-37.
3 Sharma, J., & Fulekar, M. H. (2009). Identification of potential strain-Buttiauxella Izardii Dsm 9397 for remediation of cadmium. Innovative Romanian Food Biotechnology, 4, 16-23.
4 Seo, K. J., Kim, J. M., Kim, M. J., Kim, S. K., Lee, J. E., Kim, I. Y., ... & Ko, G. P. (2009). Assessing water quality of Siheung stream in Shihwa industrial complex using both principal component analysis and multi-dimensional scaling analysis of korean water quality index and microbial community data. ?????????, 35(6).
5 Seogyeongjin, gimjumi, Kim Min Jung, Kim Sung-Keun, Lee, Ji - Eun, giminyoung ... & gogwangpyo. (2009). Principal Component Analysis and Multi-Dimensional Scaling Water quality and distribution studies of the microbial community analysis using Sihwa Industrial Complex, Siheung cloth. Korea Journal of Environmental Health, 35 (6), 454-462.
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Dr. Jaya Sharma
- India
Professor Madhusudan Fulekar
- India
mhfulekar@yahoo.com