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Inhibition of Aldose Activity by Essential Phytochemicals of Cymbopogon citratus (DC.) Stapf
Vyshali.P, K.J. Thara Saraswathi, Rajeshwari D Sanakal, B. B. Kaliwal
Pages - 257 - 267     |    Revised - 01-11-2011     |    Published - 15-12-2011
Volume - 5   Issue - 5    |    Publication Date - November / December 2011  Table of Contents
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KEYWORDS
Aldose Reductase, Molecular Docking, Citral, Geraniol, Myrcene, Cymbopogon citratus
ABSTRACT
The ambiguity of whether aldose reductase, an enzyme of polyol pathway, is linked to diabetes and its complication has been receded based on the recent studies made on the inhibition of its (Aldose reductase) activity. In our current study, we have used an in silico approach (molecular docking) to analyze the effect of essential phytochemicals obtained from Cymbopogon citratus on the aldose reductase activity. C.citratus is grown extensively in tropical countries including India for perfumery and pharmaceuticals. The essential phytochemicals of C.citratus like Myrcene, Citral, and Geraniol have been used as ligand for the molecular docking analysis with Aldose reductase as receptor. The docking analysis showed Myrcene, with binding energy of -8.76 Kcal/mol is best amongst Citral and Geraniol which are having binding energies of -7.24 Kcal/mol and -7.93 Kcal/mol respectively for inhibiting the activity of Aldose reductase.
CITED BY (9)  
1 Sheikh, B. Y. (2016). The role of prophetic medicine in the management of diabetes mellitus: A review of literature. Journal of Taibah University Medical Sciences.
2 De, B., Bhandari, K., Singla, R. K., Katakam, P., Samanta, T., Kushwaha, D. K., ... & Mitra, A. (2015). Chemometrics optimized extraction procedures, phytosynergistic blending and in vitro screening of natural enzyme inhibitors amongst leaves of Tulsi, Banyan and Jamun. Pharmacognosy Magazine, 11(44), 522.
3 Firdhouse, M. J., & Lalitha, P. (2015). Maestro 9.4 as a Tool in the Structure Based Screening of Glycoalkaloids and Related Compounds, Targeting Aldose Reductase. Trends in Bioinformatics, 8(1), 26.
4 Ezuruike, U. F., & Prieto, J. M. (2014). The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. Journal of ethnopharmacology, 155(2), 857-924.
5 Dey, B., Mitra, A., Katakam, P., & Singla, R. K. (2014). Exploration of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays. World journal of diabetes, 5(2), 209.
6 Dey, B., & Mitra, A. (2013). Chemo-profiling of eucalyptus and study of its hypoglycemic potential. World journal of diabetes, 4(5), 170.
7 Krishnamoorthy, V., Surendran, S., Sankaran, V., Vasudevan, V. P., & JARP, S. (2013). Molecular docking of secondary metabolites from Cyperus rotundus and Cyamopsis tetragonolobus against aldose reductase-A novel drug target for diabetic cataract. Journal of Pharmacy Research, 1(1).
8 Annadurai, R. S., Jayakumar, V., Mugasimangalam, R. C., Katta, M. A., Anand, S., Gopinathan, S., ... & Rao, S. N. (2012). Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties. BMC genomics, 13(1), 663.
9 Ganjewala, D., Gupta, A. K., & Muhury, R. (2012). An update on bioactive potential of a monoterpene aldehyde citral. Journal of Biologically Active Products from Nature, 2(4), 186-199.
1 Google Scholar
2 Academic Journals Database
3 CiteSeerX
4 refSeek
5 Scribd
6 SlideShare
7 PdfSR
1 T.Saraswathi, ”Regeneration of Cymbopogon citratus Stapf. and variation in essential oil composition in the regenerants”, Journal of Plant Biology, Vol. 27(1), p 47-56 , 2000
2 A. Lee and Stephen S. M. Chung Contributions of polyol pathway to oxidative stress in diabetic cataract Institute of Molecular Biology, University of Hong Kong, Pokfulam, Hong Kong, People’s Republic of China.
3 Y.Hamada, C.Nishimura, N.Koh, F.Sakakibara, J.Nakamura, T. Tanimoto “Influence of Inter individual Variability of Aldose Reductase Protein Content on Polyol-Pathway Metabolites and Redox State in Erythrocytes in Diabetic Patients”. Diabetes Care, Vol. 21(6), 1998.
4 M.Dunlop.“Aldose reductase and the role of the polyol pathway in diabetic nephropathy”. Kidney International, Vol. 58, Suppl. 77, pp. S-3–S-12, 2000.
5 A. I.G. Obrosova, P. Pacher, C. Szabo , Z. Zsengeller, H. Hirooka, M. J. Stevens, and M. A. Yorek “Aldose Reductase Inhibition Counteracts Oxidative-Nitrosative Stress and Poly(ADP- Ribose) Polymerase Activation in Tissue Sites for Diabetes Complications. Diabetes, Vol. 54, 2005.
6 S.A Sheweita, A.A Newairy, H.A. Mansour, M.I Yousef “Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxan-induced diabetic rats”. Toxicology, Vol.174(2), p131-139. 2002
7 A.A. Adeneye and E. O. Agbaje “Hypoglycemic and hypolipidemic effects of fresh leaf aqueous extract of Cymbopogon citratus Stapf. in rats”. Journal of Ethnopharmacology, Vol. 112(3), 2007.
8 G.M Morris, D.S Goodsell, R .Huey, W.E Hart, S. Halliday, R .Belew, A.J Olson. User's Guide AutoDock - Automated Docking of Flexible Ligands to Receptors Version 3.0.5. San Diego, United States of America. 2000.
9 K. H. Gabbay, “The sorbitol pathway and the complications of diabetes,” The New England Journal of Medicine, vol. 288, no. 16, p 831–836, 1973.
10 The Diabetes Control and Complications Trial Research Group, “The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus,” The New England Journal of Medicine, Vol. 329, no. 14, p 977–986, 1993.
11 American Diabetes Association, “Implications of the United Kingdom prospective diabetes study,” Diabetes Care, Vol. 22, supplement 1, p S27–S31, 1999.
12 The Writing Team for the Diabetes Control & Complications Trial/Epidemiology of Diabetes Interventions and Complications Research Group, “Effect of intensive therapy on the micro vascular complications of type 1 diabetes mellitus,” The Journal of the American Medical Association, Vol. 287, no. 19, p 2563–2569, 2002.
13 P. J. Oates, “The polyol pathway and diabetic peripheral neuropathy”, in Neurobiology of Diabetic Neuropathy, D. R. Tomlinson, Ed., Vol. 50 of International Review of Neurobiology, , Academic Press, London, UK, p 325–392,2002.
14 B. S. Szwergold, F. Kappler, and T. R. Brown, “Identification of fructose 3-phosphate in the lens of diabetic rats”, Science, Vol. 247, no. 4941, p 451–454, 1990.
15 R. G. Gonz´alez, S. Miglior, I. V. Saltza, L. Buckley, L.J.Neuringer, and H.-M. Cheng, “31P NMR studies of the diabetic lens”, Magnetic Resonance in Medicine, vol. 6, no. 4, p 435– 444, 1988.
16 P. A. Barnett, R. G. Gonz´alez, L. T. Chylack Jr., and H. - M. Cheng, “The effect of oxidation on sorbitol pathway kinetics”, Diabetes, vol. 35, no. 4, p 426–432, 1986.
17 J. R.Williamson, K. Chang, M. Frangos, et al., “Hyperglycemic pseudohypoxia and diabetic complications”, Diabetes, vol. 42, no. 6, pp. 801–813, 1993.
18 B. Lass`egue and R. E. Clempus, “Vascular NAD(P)H oxidases: specific features, expression, and regulation”, American Journal of Physiology-Regulatory Integrative and Comparative Physiology, vol. 285, no. 2, pp. R277– R297, 2003.
19 Z. Dagher, Y. S. Park, V. Asnaghi, T. Hoehn, C. Gerhardinger, and M. Lorenzi, “Studies of rat and human retinas predict a role for the polyol pathway in human diabetic retinopathy,” Diabetes, vol. 53, no. 9, pp. 2404– 411, 2004.
20 M. Lorenzi and C. Gerhardinger, “Early cellular and molecular changes induced by diabetes in the retina”, Diabetologia, vol. 44, no. 7, pp. 791–804, 2001.
21 V. Asnaghi, C. Gerhardinger, T. Hoehn, A. Adeboje, and M. Lorenzi, “A role for the polyol pathway in the early neuroretinal apoptosis and glial changes induced by diabetes in the rat”, Diabetes, vol. 52, no. 2, pp. 506–511, 2003.
22 I. G. Obrosova, A. G. Minchenko, R. Vasupuram, et al., “Aldose reductase inhibitor fidarestat prevents retinal oxidative stress and vascular endothelial growth factor overexpression in streptozotocin-diabetic rats”, Diabetes, vol. 52, no. 3, pp. 864– 871, 2003.
23 C. Distler and Z. Dreher, “Glia cells of the monkey retina- II. M¨uller cells,” Vision Research, vol. 36, no. 16, pp. 2381–2394, 1996.
24 http://dock.compbio.ucsf.edu/
25 Department of Chemistry, University of Cambridge. http://www.ch.cam.ac.uk/cil/SGTL/Tripos/
26 L.Ingber, "Simulated annealing: practice versus theory", Mathl. Comput. Modelling 18, 11, 29-57, 1993.
27 J.Renna,”Genetic Algorithm Viewer: Demonstration of a Genetic Algorithm”, Ph.D. May 2000.
28 E Tedesco, M.H Chao, G.W Turner, B.M Kariuki, R.L Johnston,”Optimisation of Lamarckian evolution in a Genetic Algorithm for structure solution from powder diffraction data”, KDM Harris School of Chemistry, University of Birmingham (UK).
29 D.K .Wilson, K.M .Bohren, K.H .Gabbay, F.A. Quiocho, “An unlikely sugar substrate site in the 1.65 A structure of the human aldose reductase holoenzyme implicated in diabetic complications”. Science, Vol. 257, p 81-84. 1992
30 Molecular Graphics Laboratory Department of Molecular Biology The Scripps Research Institute, MB-5 10550 N. Torrey Pines Rd. La Jolla, CA 92037-1000 U.S.A. http://autodock.scripps.edu/faqs-help/faq/what-is-the-format-of-a-pdbqt-file
31 http://www.rcsb.org/pdb/explore/explore.do?structureId=1ADS.
32 Advanced Chemistry Development, Inc. 110 Yonge Street, 14th floor, Toronto, Ontario, Canada M5C 1T4 http://www.acdlabs.com/products/chem_dsn_lab/chemsketch/.
33 P.F Kador, W.G Robison and J.H Kinoshita, “The pharmacology of aldose reductase inhibitors”, Ann. Rev. Pharmacol. Toxicol. Vol. 25, p 691-714. 1985.
34 M.K Kim, S.Y Kim and H.S Lee, “Rat lens aldose reductase inhibitory activities of oriental medicinal plants”, Agric. Chem. Biotechnol. Vol 45, p 84-88. 2001.
35 De la Fuente, J. A. and S. Manzanaro, Nat. Prod. Rep. Vol. 20, p 243, 2003.
36 K. Kawanishi, H. Ueda and M. Moriyasu, “Aldose reductase inhibitors from nature” Curr. Med. Chem., Vol. 10, p 1353-1374. 2003.
37 K. Sivakumari, A. Flora Mary Cyril Rathinabai, P.K. Kaleena, P. Jayaprakash and R. Srikanth. ” Molecular docking study of bark-derived components of Cinnamomum cassia on aldose reductase”, Indian Journal of Science and Technology, Vol. 3 No. 8, p 1081-1088, 2010.
38 A.A Rao, H. Thota, R.S Gumpeny, A. Akula, S.B Changalasetty, S.R Challa, T. Ravavarapu, S.P Akula, C.H Divakar, K. Srinivas and U.N Das.”Bioinformatics analysis of diabetic retinopathy using functional protein sequences”, Med. Hypotheses, Vol.70 (1), p 148-155, 2008.
Miss Vyshali.P
Department of Microbiology and Biotechnology, - India
vyshalipingale@gmail.com
Associate Professor K.J. Thara Saraswathi
Bangalore University, - India
Dr. Rajeshwari D Sanakal
Karnatak University,Dharwad - India
Professor B. B. Kaliwal
Karnatak University,Dharwad - India