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Automated Protocol for Counting Malaria Parasites (P. falciparum) from Digital Microscopic Image Based on L*a*b* Colour Model and K-Means Clustering
J. Opoku-Ansah, B. Anderson, J. M. Eghan, J. N. Boampong, P. Osei-Wusu Adueming, C. L. Y. Amuah, A. G. Akyea
Pages - 149 - 158     |    Revised - 01-10-2013     |    Published - 01-11-2013
Volume - 7   Issue - 4    |    Publication Date - November 2013  Table of Contents
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KEYWORDS
Malaria Parasites, Image processing, Segmentation, K-means, cluster, L*a*b* colour model.
ABSTRACT
Basis for malaria parasites diagnosis in most hospitals and clinics, especially in developing countries, which is manually done, is strenuous and time-consuming. In this paper, we present an automated protocol for counting malaria parasites (P. falciparum) from digital microscopic red blood cells (RBCs) mages based on L*a*b* colour model and K-Means clustering algorithm using Matlab. This method is device-independent, perceptually uniform and approximates human vision. An image slide of size 300 x 300 x 3 pixels of RBCs with malaria parasites has been counted in less than 10 seconds using a computer with 64-bit Intel (R) Celeron (R) Central Processing Unit and processing speed of 2.20 GHz. The digital counts have a good correlation with the manual counts. This automated protocol has the potential of providing fast, accurate and objective detection information for proper clinical management of patients.
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1 Opoku-Ansah, J., Eghan, M. J., Anderson, B., Boampong, J. N., & Buah-Bassuah, P. K. (2016). Laser-Induced Autofluorescence Technique for Plasmodium falciparum Parasite Density Estimation. Applied Physics Research, 8(2), 43.
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Mr. J. Opoku-Ansah
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana
Mr. B. Anderson
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana
Dr. J. M. Eghan
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana
meghan@ucc.edu.gh
Mr. J. N. Boampong
School of Biological Sciences Department of Biomedical and Forensic Sciences, University of Cape Coast, Cape Coast - Ghana
Mr. P. Osei-Wusu Adueming
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana
Mr. C. L. Y. Amuah
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana
Miss A. G. Akyea
School of Physical Sciences Department of Physics, Laser and Fibre Optics Centre (LAFOC), University of Cape Coast, Cape Coast - Ghana