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Automated Diagnosis of Malaria in Tropical Areas Using 40X Microscopic Images of Blood Smears
Kanaa Thomas Florent Noel, Tchiotsop Daniel, Ele Pierre, Tonye Emmanuel, Belong Philippe
Pages - 12 - 23     |    Revised - 31-08-2016     |    Published - 01-10-2016
Volume - 10   Issue - 2    |    Publication Date - October 2016  Table of Contents
Malaria, Plasmodium Falciparum, Median Filter, Sauvola Thresholding, Microscopic Imagery.
This paper proposes a new algorithm to measure parasitemia stemming from Plasmodium falciparum by using optical images of capillary blood smears from Sub-Saharan Africa. The approach is an improvement of the previous noteworthy method by developing a two-tone adaptive median filter and Sauvola segmentation. The analysis was performed on the database of 100X and 40X microscopic images originating from real time collection of patients' blood of some Cameroon's laboratories. The obtained results were very satisfactory with a detection of infected cells on 40X images of a sensitivity at 81.58%, a specificity at 97.11% and an accuracy at 96.71%. Compared to the previous works, the values portray a real improvement in most performance's criteria for 100X images and 40X images as well. There is a significant step of automated Malaria diagnosis in tropical areas and in the performance assessed. The results of the method gave more strength for the two magnification ranges. The time spent in analysis is obviously reduced with 40X magnification and the inaccessibility of the information by the visual laboratory measurement is henceforth genuinely assaulted. The low cost of the system opens therefore the possibility of a cost effective method of diagnosing malaria in low and middle-income countries.
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5 PdfSR 
Abdelrahman A. & Abdallah A. "Signature verification system based on support vector machine classifier." the international arab conference on information technology (acit'2013), 2013.
Anthony Moody. "Rapid Diagnostic Tests for Malaria Parasites." Clinical Microbiology Review. Vol. 15 no.1: pp 66-78, 2002.
Arash Mehrjou, Tooraj Abbasian, Maziar Izadi. "Automatic malaria diagnosis system." Robotics and Mechatronics (ICRoM), First RSI/ISM International Conference. Tehran, Iran, 2013.
C. Fourcade, M. J. C. Casbas, H. Belaouni, J. J. D. Gonzalez, P. J. J. Garcia, M. A. E. Pepio. "Automated detection of malaria by means of the haematology analyser Coulter® GEN.STM." International Journal of Laboratory Hematology, Clinical & Laboratory Haematology, Vol. 26: Issue 6, pp 367-372, 2004.
Chotivanich K., Silamut K. and Day, N. P. J. "Laboratory diagnosis of malaria infection- A short review of methods." New Zealand Journal of Medical Laboratory Science, vol. 61, pp. 4-7, 2007.
Christophe Antonio-Nkondjio, Jean Atangana, Cyrille Ndo, Parfait Awono-Ambene, Etienne Fondjo, Didier Fontenille, Frederic Simard. "Malaria transmission and rice cultivation in Lagdo, northern Cameroon." Transactions of the Royal Society of Tropical Medecine and Hygiene 102, 352-359, 2008.
Clendennen, T.E., Long, G.W. and Baird, K. J. "QBC and Giemsa stained thick blood films: diagnostic performance of laboratory technologists." Trans. R. Soc. Trop. Med. Hyg., vol. 89, issue 2: pp 183-184, 1995.
D. C. Warhurst, J. E. Williams. "Laboratory diagnosis of malaria." Journal of Clinical Pathology (J Clin Pathol) Vol.49, pp 533-538, 1996.
Daniel Maitethia. "A rapid malaria diagnostic method based on automatic detection and classification of plasmodium parasites in stained thin blood smear images", M.Sc. Thesis, the University of Nairobi, Kenya, 2014.
Dian Anggraini, Anto Satriyo Nugroho, Christian Pratama, Ismail Ekoprayitno Rozi, Vitria Pragesjvara, Made Gunawan. "Automated Status Identification of Microscopic Images Obtained from Malaria Thin Blood Smears using Bayes Decision: A study case in Plasmodium Falciparum." Proceedings of International Conference on Advanced Computer Science & Information Systems, Bandung, Indonesia, 2011.
Elizabeth A. Ashley, Khin Maung Lwin, Rose McGready, Win Htay Simon, Lucy Phaiphun, Stephane Proux, Nantawan Wangsean, Walter Taylor, Kasia Stepniewska, Wimon Nawamaneerat, Kyaw Lay Thwai, Marion Barends, Wattana Leowattana, Piero Olliaro, Pratap Singhasivanon, Nicholas J. White and Francois Nosten. "An open label randomized comparison of mefloquine- artesunate as separate tablets vs. a new co-formulated combination for the treatment of uncomplicated multidrug-resistant falciparum malaria in Thailand." Tropical Medicine and International Health - vol. 11 no 11, pp 1653-1666, 2006.
Faisal Shafait, Daniel Keysers and Thomas M. Breuel. "Efficient Implementation of Local Adaptive Thresholding Techniques Using Integral Images." Conference Proceedings SPIE, Vol. 6815, 681510, 2008.
G.O. Adeoye, I.C. Nga. "Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained thick film (GTF) for diagnosis of malaria." Elsevier Journal, Parasitology International, Vol. 56, Issue 4: pp 308-312, 2007.
Hanscheid T., Melo-Cristino J. and Pinto, B. G. "Automated detection of malaria pigment in white blood cells for the diagnosis of malaria in Portugal." American Journal of Tropical Medicine and Hygiene, vol. 64: issue 5, pp. 290-292, 2001.
Janet Cox-Singh, Timothy M. E. Davis, Kim-Sung Lee, Sunita S. G. Shamsul, Asmad Matusop, Shanmuga Ratnam, Hasan A. Rahman, David J. Conway, and Balbir Singh. "Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clinical Infectious Diseases." Vol. 46 (2), pp165-171, 2008.
M. I. Razzak. "Automatic Detection and Classification of Malarial Parasite." International Journal of Biometrics and Bioinformatics (IJBB), Vol.9: Issue 1, pp 1-12, 2015.
M.T. Makler, R.C Piper, W.K Milhous. "Lactate Dehydrogenase and the Diagnosis of Malaria." Elsevier Journal, Parasitology International, Vol. 14, Issue 9: pp 376-377, 1998.
Mamta Juneja and Rajni Mohana. "An Improved Adaptive Median Filtering Method for Impulse Noise Detection." International Journal of Recent Trends in Engineering. Vol.1(1): pp 274-278, 2009.
Milan Sonka, J. Michael Fitzpatrick. "Handbook of Medical Imaging - Medical Image Processing and Analysis." Volume 2. Bellingham, Washington: SPIE Press, USA, 2009.
Mohammad Imroze Khan, Bhibhudendra Acharya, Bikesh Kumar Singh, Jigyasa Soni. "Content Based Image Retrieval Approaches for Detection of Malarial Parasite in Blood Images." International Journal of Biometrics and Bioinformatics (IJBB), Vol. 5: Issue 2: pp 97-110, 2011.
O. Imocha Singh, Tejmani Sinam, O. James and T. Romen Singh. "Local Contrast and Mean based Thresholding Technique in Image Binarization." International Journal of Computer Applications (0975 - 8887) Vol.51, No.6, 2012.
Pawan Agrawal, Pradipti Verma. "Automated Detection and Counting of Red Blood Cell using image processing techniques." International Journal of Scientific Research and Management (IJSRM) Vol. 3 Issue 4: pp 2692-2695, 2015.
Pooja R. Patil, G. S. Sable, Gauri Anandgaonnkar. "Counting of WBCs and RBCs from blood images using gray thresholding." International Journal of Research in Engineering and Technology (IJRET), Vol. 3, Issue 4: pp 391-395, 2014.
Pramit Ghosh, Debotosh Bhattacharjee, Mita Nasipuri, Dipak Kumar Basu. "Medical Aid for Automatic Detection of Malaria." Communications in Computer and Information Science (CCIS), Vol. 245, pp 170-178, 2002.
Pranati Rakshi and Kriti Bhowmik. "Detection of presence of Parasites in Human RBC In Case of Diagnosing Malaria Using Image Processing." Proceedings of the 2013 IEEE Second International Conference on Image Information Processing (ICIIP-2013), 2013.
R. Renuka Devi, V. Rajagopal, M. Senthil Kumar and G. Magesh. "Computerized Shape Analysis Of Erythrocytes And Their Formed Aggregates In Patients Infected With P. Vivax Malaria." Advanced Computing: An International Journal (ACIJ), Vol.2, No.2, pp71-77, 2011.
Rafael C. Gonzalez, Richard E. Woods and Steven L. Eddins (2009). Digital Image Processing Using MATLAB, 2nd Edition. Gatesmark Publishing LLC.
S. S. Savkare, S. P. Narote. "Automatic Detection of Malaria Parasites for Estimating Parasitemia." International Journal of Computer Science and Security (IJCSS), Vol.5: pp 310-315, 2011.
Saad H. Abdalla and Geoffrey Pasvol. "Malaria: A Hematological Perspective." Tropical Medecine Science and Practice, Vol.4. Ed. Imperial College Faculty of Medicine, 2004,16 pgs.
Snow R.W. and Gilles H. M. "The epidemiology of malaria - Essential malariology". 4th edition. London, New York, 2002.
Stephen Y. Gbedema, Marcel T. Bayor, Kofi Annan, Colin W. Wright. "Clerodane diterpenes from Polyalthia longifolia (Sonn) Thw. var. pendula: Potential antimalarial agents for drug resistant Plasmodium falciparum infection." Journal of Ethnopharmacology 169, 176-182, 2015.
Thomas F. N. Kanaa, E. Tonye, G. Mercier, V. Onana. "Detection des nappes d'hydrocarbures dans les images RSO par morphologie mathematique." Teledetection, vol.3, pp 215-229, 2004.
World Health Organization. Bench Aids for the diagnosis of Malaria infections. WHO Global Malaria Programme, Geneva, Switzerland, 24 pgs, 2000.
World Health Organization. World Malaria Report. WHO Global Malaria Programme. World Health Organization, Geneva, Meeting of 11 - 13 Sept. 2013.
Dr. Kanaa Thomas Florent Noel
University of Bamenda - Cameroon
Associate Professor Tchiotsop Daniel
University of Dschang - Cameroon
Associate Professor Ele Pierre
University of Douala - Cameroon
Professor Tonye Emmanuel
University of Yaounde - Cameroon
Dr. Belong Philippe
University of Yaounde - Cameroon